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Monday, 22 October 2007

Overlap extension polymerase chain reaction

Overlap extension polymerase chain reaction

From Wikipedia, the free encyclopedia

The overlap extension polymerase chain reaction (OE-PCR) technique was first published in 1988 by Higuchi et al. and describes a process based on the polymerase chain reaction used to a) insert mutations at specific points in a sequence, further than ~55 nucleotides from either end and/or b) produce polynucleotides from smaller fragments.[1]

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http://en.wikipedia.org/wiki/Overlap_extension_polymerase_chain_reaction

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